Improved Cellular Analysis for the Early Detection of Compound-Induced Hepatic Cytotoxicity to Reduce Animal Testing

Most conventional cell-based in vitro assays for the assessment of cell viability and cytotoxicity are disruptive endpoint assays requiring cell lysis. A non-invasive and label-free way to continuously monitor cellular behavior can now be achieved using the xCELLigence System of Real-Time Cell Analyzers (RTCA) co-developed by Roche and ACEA Biosciences (2). xCELLigence RTCA SP, MP and DP Instruments measure electrical impedance generated when adherent cells contact the network of microelectrode sensors at the bottom of each well of the dedicated cell culture plate, E-Plate 16 (with 16 wells) and E-Plate 96 (with 96 wells). This technology allows long-term dynamic monitoring of cellular events, such as proliferation, cell death, adhesion, spreading and other morphological alterations, in real-time. Improved Cellular Analysis for the Early Detection of Compound-Induced Hepatic Cytotoxicity to Reduce Animal Testing